Effect of Physical and Chemical Mutagenesis on Grains Germination Studies, Effectiveness and Efficiency of Maize (corn) Zea Mays (L.)
Keywords:
Zea mays, LD50, Gamma rays, DES, Mutagenesis, Effectiveness and Efficiency
Abstract
In the present study was undertaken to investigate the nature of induced genetic variability in Zea mays (L.) variety sugar 75 were subjected to physical and chemical mutagenic treatments for two generations. The physical and chemical mutagens namely, gamma rays and DES (Diehthyl sulphate) was used. The mutagenic treatment seeds were tested for lethal dose 50 per cent for all mutagens, separately and the dose at which 50 per cent of the seed germination was considered as LD50 values, separately mutagenic seeds were grown in the field. In Gamma rays was found to be more effective and efficient than the other mutagens. The effectiveness was producing germination, morphological and viable mutants. The efficiency was observed based on the lethality and injury while the mutagenic effectiveness and efficiency generally decreased with increased in the higher doses of the mutagens in certain level of dose/concentrations.
References
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C.Thilagavathi and L.Mullainathan, Isolation of macro mutants and mutagenic effectiveness, efficiency in black gram (Vigna mungo (L.) Hepper), Glo. J. Mol. Sci., 4(2)(2009), 76-79.
A.S.Gautam, K.C.Sood and A.K.Richarria, Mutagenic effectiveness and efficiency of gamma rays, ethylmethane sulphonate and their synergistic effects in blackgram (Vigna mungo L.), Cytologia, 57(1992), 85-89.
J.S.Ahmed, Effectiveness and efficiency of gamma rays in blackgram, Madras Agric. J., 83(1995), 320-321.
A.J.Deepalakshmi and C.R.Anandakumar, Efficiency and effectiveness of physical and chemical mutagens in urdbean (Vigna mungo (L.) Hepper), Madras Agric. J., 90(7-9)(2003), 485-489.
S.K.Sharma, R.Sood and D.P.Pandey, Studies on mutagen sensitivity, effectiveness and efficiency in urdbean (Vigna mungo (L.) Hepper), Indian J. Genet., 65(1)(2005), 20-22.
S.Jayakumar and R.Selvaraj, Mutagenic effectiveness and efficiency of gamma rays and ethylmethane sulphonate in sunflower (Helianthus annuus L.), Madras Agric. J., 90(7-9)(2003), 574-576.
H.S.Yadava, A.N.Tikle and D.V.Bhagat, Effect of induced mutation through gamma rays on growth and yield parameters of kodo-millet, J. Soil and Crops, 13(1)(2003), 25-28.
N.P.Dubinin, Problems of radiation genetics, Oliver & Boyd, London, (1961).
Y.I.Lee, I.S.Lee and Y.P.Lim, Variation in sweet potato regenerates from gamma-rays irradiated embryogenic callus, J. Plant Biotech., 4(2002), 163-170.
K.N.Maluszynski, L.V.Zanten and B.S.Ahloowalia, Officially released mutant varieties, The FAO/IAEA Database. Mut. Breed.Rev., 12(2000), 1-12.
M.Regulski, Z.Lu, J.Kendall, M.T.Donoghue and J.Reinders, The Maize methylome influences mRNA splice sites and reveals widespread paramutation-like switches guided by small RNA, Genome Reasearch, 23(2013), 1651-1662.
J.Gent, T.F.Madzima, R.Bader, M.R.Kent and X.Zhang, Accessible DNA and relative depletion of H3K9me2 at maize loci undergoing RNA-directed DNA methylation, Plant Cell, 26(2014), 4903-4917.
P.K.Panda and P.K.Subuthi, A comparative study of chemically induced micro mutational variability in M2 generation of rape seed and mustard, Indian J. Genet., 54(3)(1994), 269-274.
C.F.Konzak, R.A.Nilan, J.Wagner and R.J.Foster, Efficient chemical mutagenesis. The use of induced mutations in plant breeding Rept, FAO/IAEA Tech. Meet. Rome., (1965).
S.Gnanamurthy, D.Dhanavel and M.Girija, Studies on Induced Chemical Mutagenesis in maize (Zea mays (L.), Inter. Journal of Curr. Science, 3(11)(2011), 037-040.
M.K.Jana, Effects of X-ray and neutron irradiation on seeds of Phaseolus mungo L, Genetia Agraria, XVIII (4)(1964), 617-628.
L.Fehr, Genetics of Vigna, In. (Ed). Horticulture Review. A. VI, Westport, Connecticut. J. Janick., (1987), 311-394.
C.Thilagavathi, Effect of Ethyl methane sulphonate (EMS) and Di ethyl sulphonate (DES) in black gram, Vigna mungo (L.) Hepper, M.Phil. Thesis, Annamalai University, Annamalainagar, Tamil Nadu, (2007).
S.Ignacimuthu and C.R.Babu, Radio sensitivity of the wild and cultivated urd and mungbean, Indian J. Genet., 48(1988), 331-342.
P.C.Balakrishnan, Induced mutagenesis in soybean (Glycine max (L.) Merill), Ph.D. Thesis, Tamil Nadu Agrl. Univ., Coimbatore, (1991).
K.Geetha, Studies on induced mutation in two distinct varieties of soybean (Glycine max (L.) Merill), Ph.D. Thesis, Tamil Nadu Agrl. Univ., Coimbatore, (1994).
P.Pavadai and D.Dhanavel, Effect of EMS, DES and colchicine treatment in soybean, Crop Res., 28(1-3)(2004), 118-120.
S.Velu, L.Mullainathan, D.Arulbalachandran, D.Dhanavel and R.Poonguzhali, Effectiveness and Efficiency of gamma rays and EMS on cluster bean [Cyamopsis tetragonoloba (L.) Taub.], Crop Res., 34(1-3)(2007), 249-251.
M.Girija and D.Dhanavel, Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combined treatment in cowpea (Vigna unguiculata L. Walp), Glo. J. Mol. Sci, 4(2)(2009), 68-75.
H.Gaul, Mutagenic effects observed in the first generation. I. Pant injury and Lethality. II. Cytological effects. III. Sterility. Manual on Mutation Breeding, IAEA, Vienna, (1970), 85-89.
D.Kavithamani, A.Kalamani, C.Vanniarajan and D.Uma, Mutagenic effectiveness and efficiency of gamma rays and EMS in soybean (Glycine max (L.) Merrill), Madras Agric. J., 95(7-12)(2008), 448-451.
A.S.Gautam, K.C.Sood and R.K.Mittal, Mutagenic effectiveness and efficiency of gamma rays and ethylmethane sulphonate in rajmash (Phaseolus vulgaris L.), Legume Res., 21(3-4)(1998), 217-220.
I.S.Solanki, Isolation of macromutations and mutagenic effectiveness and efficiency in lentil (Lens culinaris Medik.), Indian J. Genet., 65(4)(2005), 264-268.
D.Sassikumar, T.Nepolean and A.Gopalan, Effectiveness and efficiency of the mutagens gamma rays and ethylmethane sulphonate on lima bean (Phaseolus lunatus L.), Indian J. Agric. Res., 37(2)(2003), 115-119.
C.Thilagavathi and L.Mullainathan, Isolation of macro mutants and mutagenic effectiveness, efficiency in black gram (Vigna mungo (L.) Hepper), Glo. J. Mol. Sci., 4(2)(2009), 76-79.
A.S.Gautam, K.C.Sood and A.K.Richarria, Mutagenic effectiveness and efficiency of gamma rays, ethylmethane sulphonate and their synergistic effects in blackgram (Vigna mungo L.), Cytologia, 57(1992), 85-89.
J.S.Ahmed, Effectiveness and efficiency of gamma rays in blackgram, Madras Agric. J., 83(1995), 320-321.
A.J.Deepalakshmi and C.R.Anandakumar, Efficiency and effectiveness of physical and chemical mutagens in urdbean (Vigna mungo (L.) Hepper), Madras Agric. J., 90(7-9)(2003), 485-489.
S.K.Sharma, R.Sood and D.P.Pandey, Studies on mutagen sensitivity, effectiveness and efficiency in urdbean (Vigna mungo (L.) Hepper), Indian J. Genet., 65(1)(2005), 20-22.
S.Jayakumar and R.Selvaraj, Mutagenic effectiveness and efficiency of gamma rays and ethylmethane sulphonate in sunflower (Helianthus annuus L.), Madras Agric. J., 90(7-9)(2003), 574-576.
H.S.Yadava, A.N.Tikle and D.V.Bhagat, Effect of induced mutation through gamma rays on growth and yield parameters of kodo-millet, J. Soil and Crops, 13(1)(2003), 25-28.
How to Cite
P. Rajapandian, & D. Dhanam. (2018). Effect of Physical and Chemical Mutagenesis on Grains Germination Studies, Effectiveness and Efficiency of Maize (corn) Zea Mays (L.). International Journal of Current Research in Science and Technology, 4(3), 1-7. Retrieved from https://crst.gfer.org/index.php/crst/article/view/106
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